UmiCorrectionMetrics
Metrics tracking how well observed UMIs match expected UMI sequences.
These metrics are generated per-UMI and track the distribution of match types (perfect matches, single mismatches, etc.) for each expected UMI.
Fields
umi- The expected/corrected UMI sequence (or all Ns for unmatched)total_matches- Total UMI sequences matched/corrected to this UMIperfect_matches- Number of reads with zero mismatchesone_mismatch_matches- Number of reads with exactly one mismatchtwo_mismatch_matches- Number of reads with exactly two mismatchesother_matches- Number of reads with three or more mismatchesfraction_of_matches- Proportion of all reads matching this UMIrepresentation- Ratio of this UMI’s count to the mean count across all UMIs
Fields
| Column | Type | Description |
|---|---|---|
umi | String | The corrected UMI sequence (or all Ns for unmatched). |
total_matches | u64 | The number of UMI sequences that matched/were corrected to this UMI. |
perfect_matches | u64 | The number of UMI sequences that were perfect matches to this UMI. |
one_mismatch_matches | u64 | The number of UMI sequences that matched with a single mismatch. |
two_mismatch_matches | u64 | The number of UMI sequences that matched with two mismatches. |
other_matches | u64 | The number of UMI sequences that matched with three or more mismatches. |
fraction_of_matches | f64 | The fraction of all UMIs that matched or were corrected to this UMI. |
representation | f64 | The total_matches for this UMI divided by the mean total_matches for all UMIs. |